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CBX1 Recombinant antibody

CBX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240075E1

Cat no : 83248-3-RR

Print datasheet

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

验证数据展示

  • WB analysis using 83248-3-RR

    WB analysis using 83248-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-3-RR (CBX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83248-3-RR

    IF Staining of HeLa using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83248-3-RR

    IF Staining of HeLa using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of A431 using 83248-3-RR

    IF Staining of A431 using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of A431 using 83248-3-RR

    IF Staining of A431 using 83248-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83248-3-RR

    FC experiment of HeLa using 83248-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CBX1 Recombinant antibody (83248-3-RR, Clone:240075E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83248-3-RR

    Affinity and Kinetic Characterization of 83248-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-3-RR against Human CBX1 were performed. The affinity constant is 3.31 nM.

经过测试的应用

Positive WB detected inCHO cells, PC-12 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells, A431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83248-3-RR is CBX1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CBX1 fusion protein Ag34901 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Synonyms HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

实验方案

Product Specific Protocols
WB protocol for CBX1 antibody 83248-3-RRDownload protocol
IF protocol for CBX1 antibody 83248-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols