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CBX1 Recombinant antibody, PBS Only

CBX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240075E1

Cat no : 83248-3-PBS

Print datasheet

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

验证数据展示

  • WB analysis using 83248-3-RR (same clone as 83248-3-PBS)

    WB analysis using 83248-3-RR (same clone as 83248-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-3-RR (CBX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

    IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

    IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    FC experiment of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CBX1 Recombinant antibody (83248-3-RR, Clone:240075E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83248-3-RR

    Affinity and Kinetic Characterization of 83248-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-3-RR against Human CBX1 were performed. The affinity constant is 3.31 nM.

Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

产品信息

83248-3-PBS targets CBX1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen CBX1 fusion protein Ag34901 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Synonyms HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX
Calculated Molecular Weight 27 kDa
GenBank Accession NumberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C