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验证数据展示

  • WB analysis of NIH/3T3 using 66446-1-Ig (same clone as 66446-1-PBS)

    WB analysis of NIH/3T3 using 66446-1-Ig (same clone as 66446-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66446-1-Ig (same clone as 66446-1-PBS)

    IF Staining of HepG2 using 66446-1-Ig (same clone as 66446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

CBX3 Monoclonal antibody, PBS Only

CBX3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66446-1-PBS

产品说明书

CloneNo. 1A5A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CBX3 Ready-To-Use IHC Kit. CBX3 primary antibody included.

产品信息

66446-1-PBS targets CBX3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CBX3 fusion protein Ag19112 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 chromobox homolog 3 (HP1 gamma homolog, Drosophila)
别名 CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein
计算分子量 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000954
基因名称 CBX3
Gene ID (NCBI) 11335
RRIDAB_2881815
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13185
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins