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CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A3

Cat no : 66446-1-Ig

Print datasheet

Synonyms

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

验证数据展示

  • WB analysis of NIH/3T3 using 66446-1-Ig

    WB analysis of NIH/3T3 using 66446-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66446-1-Ig

    IF Staining of HepG2 using 66446-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66446-1-Ig targets CBX3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CBX3 fusion protein Ag19112 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Synonyms CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000954
Gene Symbol CBX3
Gene ID (NCBI) 11335
RRIDAB_2881815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13185
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

实验方案

Product Specific Protocols
WB protocol for CBX3 antibody 66446-1-IgDownload protocol
IF protocol for CBX3 antibody 66446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Authors - Dan Wang
View Article