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验证数据展示

  • FC experiment of HeLa using CL647-66446

    FC experiment of HeLa using CL647-66446

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CBX3 (CL647-66446, Clone:1A5A3) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for FC (Intra)
Cat No. CL647-66446

产品说明书

CloneNo. 1A5A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

FC (Intra)

别名

CBX3, HECH, HP1 gamma, HP1Hs gamma, Modifier 2 protein, 1A5A3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CBX3 Ready-To-Use IHC Kit. CBX3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66446-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66446 targets CBX3 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 CBX3 fusion protein Ag19112 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 chromobox homolog 3 (HP1 gamma homolog, Drosophila)
别名 CBX3, HECH, HP1 gamma, HP1Hs gamma, Modifier 2 protein, 1A5A3
计算分子量 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000954
基因名称 CBX3
Gene ID (NCBI) 11335
RRIDAB_2935054
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13185
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CBX3 antibody CL647-66446Download protocol
Standard Protocols
Click here to view our Standard Protocols