CoraLite® Plus 647-conjugated CBX3 Monoclonal antibody
CBX3 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1A5A3
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66446-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66446 targets CBX3 in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | CBX3 fusion protein Ag19112 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | chromobox homolog 3 (HP1 gamma homolog, Drosophila) |
Synonyms | CBX3, HECH, HP1 gamma, HP1Hs gamma, Modifier 2 protein, 1A5A3 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC000954 |
Gene Symbol | CBX3 |
Gene ID (NCBI) | 11335 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13185 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CBX3 antibody CL647-66446 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |