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CoraLite® Plus 488-conjugated CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5A3

Cat no : CL488-66446

Print datasheet

Synonyms

Modifier 2 protein, HP1Hs gamma, HP1 gamma, HECH, 1A5A3

验证数据展示

  • IF Staining of HepG2 using CL488-66446

    IF Staining of HepG2 using CL488-66446

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CBX3 antibody (CL488-66446, Clone: 1A5A3 ) at dilution of 1:500.

  • FC experiment of HeLa using CL488-66446

    FC experiment of HeLa using CL488-66446

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBX3 (CL488-66446, Clone:1A5A3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66446-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66446 targets CBX3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen CBX3 fusion protein Ag19112 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Synonyms Modifier 2 protein, HP1Hs gamma, HP1 gamma, HECH, 1A5A3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000954
Gene Symbol CBX3
Gene ID (NCBI) 11335
RRIDAB_2883338
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13185
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CBX3 antibody CL488-66446Download protocol
FC protocol for CL Plus 488 CBX3 antibody CL488-66446Download protocol
Standard Protocols
Click here to view our Standard Protocols