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CBX5 Recombinant antibody, PBS Only (Detector)

CBX5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240145F11

Cat no : 83258-5-PBS

Print datasheet

Synonyms

HP1A, HP1 alpha, HP1, Antigen p25, 240145F11

验证数据展示

  • WB analysis using 83258-5-RR (same clone as 83258-5-PBS)

    WB analysis using 83258-5-RR (same clone as 83258-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83258-5-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of human placenta using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of human placenta using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of human placenta using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of mouse brain using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of mouse brain using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of mouse stomach using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of mouse stomach using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of rat kidney using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of rat kidney using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of rat kidney using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of rat kidney using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of rat stomach using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of rat stomach using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IHC staining of rat stomach using 83258-5-RR (same clone as 83258-5-PBS)

    IHC staining of rat stomach using 83258-5-RR (same clone as 83258-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 83258-5-RR (CBX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBX5 antibody included
  • IF Staining of HepG2 using 83258-5-RR (same clone as 83258-5-PBS)

    IF Staining of HepG2 using 83258-5-RR (same clone as 83258-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-5-RR, Clone: 240145F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83258-5-RR (same clone as 83258-5-PBS)

    FC experiment of SH-SY5Y using 83258-5-RR (same clone as 83258-5-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug CBX5 Recombinant antibody (83258-5-RR, Clone:240145F11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00181-3

    Cytometric bead array standard curve of MP00181-3

    Cytometric bead array standard curve of MP00181-3, CBX5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83258-2-PBS. Detection antibody: 83258-5-PBS. Standard: Ag34810. Range: 3.125-100 ng/mL.

  • Affinity and Kinetic Characterization of 83258-5-PBS

    Affinity and Kinetic Characterization of 83258-5-PBS

    Biolayer interferometry (BLl) kinetic assay of 83258-5-PBS against Human CBX5 was performed. The affinity constant is 0.521 nM.

  • Affinity and Kinetic Characterization of 83258-5-RR

    Affinity and Kinetic Characterization of 83258-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83258-5-RR against Human CBX5 were performed. The affinity constant is 0.339 nM.

Planning an IHC experiment? We recommend our IHCeasy CBX5 Ready-To-Use IHC Kit. CBX5 primary antibody included.

产品信息

83258-5-PBS targets CBX5 as part of a matched antibody pair:

MP00181-3: 83258-2-PBS capture and 83258-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CBX5 fusion protein Ag34810 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Synonyms HP1A, HP1 alpha, HP1, Antigen p25, 240145F11
Calculated Molecular Weight 191 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC006821
Gene Symbol CBX5
Gene ID (NCBI) 23468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45973
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443).