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CBX5 Recombinant antibody, PBS Only (Detector)

CBX5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240145F11

Cat no : 83258-5-PBS

Print datasheet

Synonyms

HP1A, HP1 alpha, HP1, Antigen p25, 240145F11

验证数据展示

  • WB analysis using 83258-5-RR (same clone as 83258-5-PBS)

    WB analysis using 83258-5-RR (same clone as 83258-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83258-5-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83258-5-RR (same clone as 83258-5-PBS)

    IF Staining of HepG2 using 83258-5-RR (same clone as 83258-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-5-RR, Clone: 240145F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83258-5-RR (same clone as 83258-5-PBS)

    FC experiment of SH-SY5Y using 83258-5-RR (same clone as 83258-5-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug CBX5 Recombinant antibody (83258-5-RR, Clone:240145F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00181-3

    Cytometric bead array standard curve of MP00181-3

    Cytometric bead array standard curve ofMP00181-3, CBX5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83258-2-PBS. Detection antibody: 83258-5-PBS. Standard: Ag34810. Range: 3.125-100 ng/mL.

  • Affinity and Kinetic Characterization of 83258-5-RR

    Affinity and Kinetic Characterization of 83258-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83258-5-RR against Human CBX5 were performed. The affinity constant is 0.339 nM.

Planning an IHC experiment? We recommend our IHCeasy CBX5 Ready-To-Use IHC Kit. CBX5 primary antibody included.

产品信息

83258-5-PBS targets CBX5 as part of a matched antibody pair:

MP00181-3: 83258-2-PBS capture and 83258-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CBX5 fusion protein Ag34810 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Synonyms HP1A, HP1 alpha, HP1, Antigen p25, 240145F11
Calculated Molecular Weight 191 aa, 22 kDa
GenBank Accession NumberBC006821
Gene Symbol CBX5
Gene ID (NCBI) 23468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45973
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C