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验证数据展示

  • IF Staining of HeLa using CL594-66497

    IF Staining of HeLa using CL594-66497

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated CCAR2 antibody (CL594-66497, Clone: 2D10F8 ) at dilution of 1:100.

  • FC experiment of U2OS using CL594-66497

    FC experiment of U2OS using CL594-66497

    1X10^6 U2OS cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CCAR2 Monoclonal antibody

CCAR2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66497

产品说明书

CloneNo. 2D10F8

宿主/亚型

Mouse / IgG2a

种属反应性

Human, pig

应用

IF/ICC, FC (Intra)

别名

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells, HeLa cells
Positive FC detected inU2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy DBC1/CCAR2 Ready-To-Use IHC Kit. DBC1/CCAR2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66497-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66497 targets CCAR2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, pig
免疫原 CCAR2 fusion protein Ag18374 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA1967
别名 CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967
计算分子量 923 aa, 103 kDa
GenBank蛋白编号BC018269
基因名称 CCAR2
Gene ID (NCBI) 57805
RRIDAB_2883597
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N163
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

实验方案

Product Specific Protocols
IF protocol for CL594 CCAR2 antibody CL594-66497Download protocol
Standard Protocols
Click here to view our Standard Protocols