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验证数据展示

  • WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

    WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 29824-1-AP (same clone as 29824-1-PBS)

    WB analysis of HepG2 using 29824-1-AP (same clone as 29824-1-PBS)

    WB result of CCDC111/PRIMPOL antibody (29824-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC111/PRIMPOL transfected HepG2 cells. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

    WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 29824-1-AP (same clone as 29824-1-PBS)

    IHC staining of human kidney using 29824-1-AP (same clone as 29824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 29824-1-AP (same clone as 29824-1-PBS)

    IF Staining of U2OS using 29824-1-AP (same clone as 29824-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC111/PRIMPOL antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CCDC111/PRIMPOL Polyclonal antibody, PBS Only

CCDC111/PRIMPOL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29824-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29824-1-PBS targets CCDC111/PRIMPOL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CCDC111/PRIMPOL fusion protein Ag31478 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 111
别名
观测分子量64 kDa
GenBank蛋白编号BC064600
基因名称 CCDC111/PRIMPOL
Gene ID (NCBI) 201973
RRIDAB_2918349
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LW4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).