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验证数据展示

  • WB analysis of Neuro-2a using 68112-1-Ig

    WB analysis of Neuro-2a using 68112-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse eye using 68112-1-Ig

    IF Staining of mouse eye using 68112-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse eye using 68112-1-Ig

    IF Staining of mouse eye using 68112-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 68112-1-Ig

    WB analysis using 68112-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CEP89, CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • WB analysis using 68112-1-Ig

    WB analysis using 68112-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 68112-1-Ig

    IF Staining of hTERT-RPE1 using 68112-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CoraLite®594 ARL13B antibody (CL594-17711, red).

CEP89, CCDC123 Monoclonal antibody

CEP89, CCDC123 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68112-1-Ig

产品说明书

CloneNo. 1F12C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

CEP89, CCDC123, 1F12C5, Cep123, Centrosomal protein of 89 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Neuro-2a cells, A431 cells, SH-5Y5Y cells, U-251 cells, SH-SY5Y cells
Positive IF-P detected inmouse eye tissue, hTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68112-1-Ig targets CEP89, CCDC123 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 CEP89, CCDC123 fusion protein Ag28339 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil domain containing 123
别名 CEP89, CCDC123, 1F12C5, Cep123, Centrosomal protein of 89 kDa
计算分子量 783 aa, 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC136328
基因名称 CEP89
Gene ID (NCBI) 84902
RRIDAB_2923641
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96ST8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).

实验方案

Product Specific Protocols
WB protocol for CEP89, CCDC123 antibody 68112-1-IgDownload protocol
IF protocol for CEP89, CCDC123 antibody 68112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols