• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CCDC68 Polyclonal antibody

CCDC68 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26301-1-AP

Print datasheet

Synonyms

CCDC68, CTCL associated antigen se57 1, SE57 1

验证数据展示

  • WB analysis of A431 using 26301-1-AP

    WB analysis of A431 using 26301-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 26301-1-AP (CCDC68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 26301-1-AP

    IHC staining of human testis using 26301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26301-1-AP (CCDC68 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 26301-1-AP

    IHC staining of human testis using 26301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26301-1-AP (CCDC68 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA431 cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

26301-1-AP targets CCDC68 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CCDC68 fusion protein Ag23622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 68
Synonyms CCDC68, CTCL associated antigen se57 1, SE57 1
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC029508
Gene Symbol CCDC68
Gene ID (NCBI) 80323
RRIDAB_2880470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2F9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled-coil domain containing 68 (CCDC68) demonstrates a tumor-suppressive role in pancreatic ductal adenocarcinoma. (PMID: 25381825)

实验方案

Product Specific Protocols
WB protocol for CCDC68 antibody 26301-1-APDownload protocol
IHC protocol for CCDC68 antibody 26301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Elife

Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages.

Authors - Weng Man Chong
View Article
humanIF

Adv Sci (Weinh)

CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC

Authors - Qi Li
View Article