CoraLite® Plus 488-conjugated CCDC94 Recombinant antibody
CCDC94 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240517E9
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
For other applications, we recommend the unconjugated version of this antibody, 83580-5-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83580-5 is CCDC94 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | CCDC94 fusion protein Ag13764 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | coiled-coil domain containing 94 |
| Synonyms | YJU2, Coiled-coil domain-containing protein 94, 240517E9 |
| Calculated Molecular Weight | 323 aa, 37 kDa |
| GenBank Accession Number | BC000561 |
| Gene Symbol | CCDC94 |
| Gene ID (NCBI) | 55702 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BW85 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CCDC94 antibody CL488-83580-5 | Download protocol |
| FC protocol for CL Plus 488 CCDC94 antibody CL488-83580-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |