验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21869-1-AP targets CCHCR1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CCHCR1 fusion protein Ag16141 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil alpha-helical rod protein 1 |
| 别名 | Putative gene 8 protein, Pg8, HCR, Coiled-coil alpha-helical rod protein 1, C6orf18 |
| 计算分子量 | 871 aa, 99 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC110534 |
| 基因名称 | CCHCR1 |
| Gene ID (NCBI) | 54535 |
| RRID | AB_2878932 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TD31 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCHCR1, also named as C6orf18 or Putative gene 8 protein, is a 782 amino acid protein, which is a nuclear protein abundantly expressed in heart, liver, skeletal muscle, kidney and pancreas, and to a lesser extent in lung and placenta. HCR is overexpressed in keratinocytes of psoriatic lesions. HCR is associated with susceptibility to psoriasis, a chronic inflammatory dermatosis that affects approximately 2% of the population. CCHCR1 may be a regulator of keratinocyte proliferation or differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCHCR1 antibody 21869-1-AP | Download protocol |
| IF protocol for CCHCR1 antibody 21869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
