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  • Featured Product
  • KD/KO Validated

Cyclin A2 Polyclonal antibody

Cyclin A2 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18202-1-AP

Print datasheet

Synonyms

CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA

验证数据展示

  • WB analysis using 18202-1-AP

    WB analysis using 18202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18202-1-AP

    WB analysis of HeLa using 18202-1-AP

    WB result of Cyclin A2 antibody (18202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.

  • IHC staining of human tonsillitis using 18202-1-AP

    IHC staining of human tonsillitis using 18202-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IHC staining of human tonsillitis using 18202-1-AP

    IHC staining of human tonsillitis using 18202-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IF Staining of HepG2 using 18202-1-AP

    IF Staining of HepG2 using 18202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin A2 antibody (18202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of MCF-7 using 18202-1-AP

    IF Staining of MCF-7 using 18202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin A2 antibody (18202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 18202-1-AP

    FC experiment of HeLa using 18202-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin A2 (18202-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IP experiment of HL-60 using 18202-1-AP

    IP experiment of HL-60 using 18202-1-AP

    IP result of anti-Cyclin A2 (IP:18202-1-AP, 3ug; Detection:18202-1-AP 1:500) with HL-60 cells lysate 4000ug.

  • IP experiment of HeLa using 18202-1-AP

    IP experiment of HeLa using 18202-1-AP

    IP result of anti-Cyclin A2 (IP:18202-1-AP, 4ug; Detection:18202-1-AP 1:20000) with HeLa cells lysate 1320 ug.

经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells, U-937 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inHeLa cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18202-1-AP targets Cyclin A2 in WB, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, monkey
Immunogen Cyclin A2 fusion protein Ag12765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin A2
Synonyms CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA
Calculated Molecular Weight 432 aa, 49 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC104783
Gene Symbol Cyclin A2
Gene ID (NCBI) 890
RRIDAB_10597084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20248
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.

实验方案

Product Specific Protocols
WB protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IHC protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IF protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IP protocol for Cyclin A2 antibody 18202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

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Authors - Xiaoqian Ji
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mouseWB

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Mol Cell

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humanWB

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CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

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mouse

Acta Pharm Sin B

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

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humanWB

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The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis

Authors - Zhenlong Yu
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