- Featured Product
- KD/KO Validated
Cyclin A2 Polyclonal antibody
Cyclin A2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
120
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HeLa cells, U-937 cells, NIH/3T3 cells, MCF-7 cells, K-562 cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
产品信息
18202-1-AP targets Cyclin A2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | CoIP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey, pig |
| Immunogen | Cyclin A2 fusion protein Ag12765 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin A2 |
| Synonyms | CCN1, CCNA, CCNA2, Cyclin A, cyclin A2, cyclinA2 |
| Calculated molecular weight | 432 aa, 49 kDa |
| Observed molecular weight | 48-52 kDa |
| GenBank accession number | BC104783 |
| Gene symbol | CCNA2 |
| Uniprot | P20248 |
| Gene ID (NCBI) | 890 |
| RRID | AB_10597084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
| IHC protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
| IF protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
| IP protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. | ||
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
Int J Biol Sci ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway | ||
Cell Death Dis HDAC7/c-Myc signaling pathway promotes the proliferation and metastasis of choroidal melanoma cells | ||
Clin Transl Med Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes |
