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验证数据展示

  • IF Staining of HeLa using CL488-18202

    IF Staining of HeLa using CL488-18202

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Cyclin A2 antibody (CL488-18202) at dilution of 1:100.

  • FC experiment of HeLa using CL488-18202

    FC experiment of HeLa using CL488-18202

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cyclin A2 (CL488-18202) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cyclin A2 Polyclonal antibody

Cyclin A2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-18202

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18202-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18202 targets Cyclin A2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Cyclin A2 fusion protein Ag12765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin A2
Synonyms CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA
Calculated Molecular Weight 432 aa, 49 kDa
GenBank Accession NumberBC104783
Gene Symbol Cyclin A2
Gene ID (NCBI) 890
RRIDAB_2919157
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20248
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin A2 antibody CL488-18202Download protocol
Standard Protocols
Click here to view our Standard Protocols