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CCNI2 Polyclonal antibody

CCNI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18822-1-AP

Print datasheet

Synonyms

CCNI2, cyclin I family, member 2, Cyclin I2

验证数据展示

  • WB analysis using 18822-1-AP

    WB analysis using 18822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 18822-1-AP

    IHC staining of human lung cancer using 18822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 18822-1-AP (CCNI2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 18822-1-AP

    IHC staining of human lung cancer using 18822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 18822-1-AP (CCNI2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18822-1-AP targets CCNI2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin I family, member 2
Synonyms CCNI2, cyclin I family, member 2, Cyclin I2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC144417
Gene Symbol CCNI2
Gene ID (NCBI) 645121
RRIDAB_10596930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZMN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCNI2 belongs to the cyclin family.

实验方案

Product Specific Protocols
WB protocol for CCNI2 antibody 18822-1-APDownload protocol
IHC protocol for CCNI2 antibody 18822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Sci Rep

Cyclin I-like (CCNI2) is a cyclin-dependent kinase 5 (CDK5) activator and is involved in cell cycle regulation.

Authors - Chengcheng Liu
View Article