Cyclin T1 Polyclonal antibody
Cyclin T1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, Jurkat cells, K-562 cells, Y79 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
20992-1-AP targets Cyclin T1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin T1 |
| Synonyms | CCNT1, CCNT, CYCT1, Cyclin-T1, Cyclin-T |
| Calculated Molecular Weight | 81 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | NM_001240 |
| Gene Symbol | Cyclin T1 |
| Gene ID (NCBI) | 904 |
| RRID | AB_10950894 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60563 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin T1 antibody 20992-1-AP | Download protocol |
| IHC protocol for Cyclin T1 antibody 20992-1-AP | Download protocol |
| IF protocol for Cyclin T1 antibody 20992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters. | ||
BMC Cancer The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study |
