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Cyclin T1 Polyclonal antibody

Cyclin T1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20992-1-AP

Print datasheet

Synonyms

CCNT, CCNT1, Cyclin T, cyclin T1, CYCT1, HIVE1

验证数据展示

  • WB analysis using 20992-1-AP

    WB analysis using 20992-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 20992-1-AP

    WB analysis of K-562 using 20992-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 20992-1-AP

    IF Staining of MCF-7 using 20992-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin T1 antibody (20992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, Y79 cells, A431 cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20992-1-AP targets Cyclin T1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin T1
Synonyms CCNT, CCNT1, Cyclin T, cyclin T1, CYCT1, HIVE1
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberNM_001240
Gene Symbol CCNT1
Gene ID (NCBI) 904
RRIDAB_10950894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60563
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

实验方案

Product Specific Protocols
WB protocol for Cyclin T1 antibody 20992-1-APDownload protocol
IF protocol for Cyclin T1 antibody 20992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
View Article
humanWB

Nucleic Acids Res

HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.

Authors - Sebastian Eilebrecht
View Article
humanWB,IF

BMC Cancer

The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study

Authors - Ying E
View Article