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CCR5 Recombinant antibody

CCR5 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230266A6

Cat no : 82942-1-RR

Print datasheet

Synonyms

C-C CKR-5, CC CKR 5, C-C chemokine receptor type 5, C C CKR 5, C C chemokine receptor type 5

验证数据展示

  • WB analysis using 82942-1-RR

    WB analysis using 82942-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82942-1-RR (CCR5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82942-1-RR

    Affinity and Kinetic Characterization of 82942-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82942-1-RR against Human CCR5 were performed. The affinity constant is 0.921 nM.

经过测试的应用

Positive WB detected inU-937 cells, mouse thymus tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82942-1-RR targets CCR5 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen CCR5 fusion protein Ag11493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) receptor 5
Synonyms C-C CKR-5, CC CKR 5, C-C chemokine receptor type 5, C C CKR 5, C C chemokine receptor type 5
Calculated Molecular Weight 352 aa, 41 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC038398
Gene Symbol CCR5
Gene ID (NCBI) 1234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic viruses, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2, and RANTES.

实验方案

Product Specific Protocols
WB protocol for CCR5 antibody 82942-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols