验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | Jurkat cells, human tonsillitis tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
IF | See 1 publications below |
产品信息
66801-1-Ig targets CCR6 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with Human samples.
经测试应用 | IHC, IF/ICC, IF-P, ELISA Application Description |
文献引用应用 | IF, IHC |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | CCR6 fusion protein Ag24195 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | chemokine (C-C motif) receptor 6 |
别名 | BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22 |
计算分子量 | 42 kDa |
GenBank蛋白编号 | BC037960 |
基因名称 | CCR6 |
Gene ID (NCBI) | 1235 |
RRID | AB_2882144 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P51684 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR6, also known as CD196, is a C-C chemokine receptor that belongs to family A of G protein-coupled receptor superfamily. It is expressed in most B cells, memory T cells, and some subsets of dendritic cells (PMID: 17057190). CCR6 is a receptor for the C-C type chemokine CCL20 (PMID: 9169459). The ligand-receptor pair CCL20-CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T-cells, and B-cells and plays a role in skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and rheumatoid arthritis (PMID: 12948524). CCR6 polymorphism has been associated with rheumatoid arthritis susceptibility and CCR6 is involved in IL-17-driven autoimmunity in human diseases (PMID: 20453841).
实验方案
Product Specific Protocols | |
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IHC protocol for CCR6 antibody 66801-1-Ig | Download protocol |
IF protocol for CCR6 antibody 66801-1-Ig | Download protocol |
FC protocol for CCR6 antibody 66801-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Neoplasia Gut microbiota-derived short-chain fatty acids promote prostate cancer progression via inducing cancer cell autophagy and M2 macrophage polarization | ||
Cell Death Dis RANKL/RANK signaling recruits Tregs via the CCL20-CCR6 pathway and promotes stemness and metastasis in colorectal cancer |