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CCT2 Polyclonal antibody

CCT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24896-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (2)

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, NIH/3T3 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CCT2 Ready-To-Use IHC Kit. CCT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24896-1-AP targets CCT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CCT2 fusion protein Ag19264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 2 (beta)
别名 PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta
计算分子量 535 aa, 57 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC113516
基因名称 CCT2
Gene ID (NCBI) 10576
RRIDAB_2879783
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78371
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

实验方案

Product Specific Protocols
WB protocol for CCT2 antibody 24896-1-APDownload protocol
IHC protocol for CCT2 antibody 24896-1-APDownload protocol
IF protocol for CCT2 antibody 24896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Authors - Yu-Cheng Zhang
humanWB

Pharmacol Res

Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.

Authors - Jiale Zhu
  • KD Validated
humanWB

PLoS One

Proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp.

Authors - Dandan Ma
mouseIF

Signal Transduct Target Ther

Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

Authors - Boyuan Ma
humanWB

Autophagy

Copper-dependent autophagic degradation of GPX4 drives ferroptosis

Authors - Qian Xue
humanWB

Mol Cell

Genome-scale exon perturbation screens uncover exons critical for cell fitness

Authors - Mei-Sheng Xiao
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