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验证数据展示

  • IF Staining of NIH/3T3 using CL488-24896

    IF Staining of NIH/3T3 using CL488-24896

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 CCT2 antibody (CL488-24896) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-24896

    FC experiment of NIH/3T3 using CL488-24896

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cct2 Polyclonal Antibody (CL488-24896)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CCT2 Polyclonal antibody

CCT2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-24896

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CCT2 Ready-To-Use IHC Kit. CCT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24896-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24896 targets CCT2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 CCT2 fusion protein Ag19264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 2 (beta)
别名 PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta
计算分子量 535 aa, 57 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC113516
基因名称 CCT2
Gene ID (NCBI) 10576
RRIDAB_3672779
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78371
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT2 is a gene encoding a molecular chaperone member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CCT2 antibody CL488-24896Download protocol
FC protocol for CL Plus 488 CCT2 antibody CL488-24896Download protocol
Standard Protocols
Click here to view our Standard Protocols