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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68214-1-Ig (same clone as 68214-1-PBS)

    WB analysis using 68214-1-Ig (same clone as 68214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 68214-1-Ig (same clone as 68214-1-PBS)

    IF Staining of NIH/3T3 using 68214-1-Ig (same clone as 68214-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

CCT2 Monoclonal antibody, PBS Only

CCT2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68214-1-PBS

产品说明书

CloneNo. 2H6C7

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Pig, Rabbit, Rat

应用

WB, IF/ICC, ELISA

别名

99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CCT2 Ready-To-Use IHC Kit. CCT2 primary antibody included.

产品信息

68214-1-PBS targets CCT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rabbit, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Pig, Rabbit, Rat
免疫原 CCT2 fusion protein Ag19264 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 2 (beta)
别名 99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta
计算分子量 535 aa, 57 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC113516
基因名称 CCT2
Gene ID (NCBI) 10576
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78371
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)