验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy CCT2 Ready-To-Use IHC Kit. CCT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68214-1-Ig targets CCT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rabbit, Rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Pig, Rabbit, Rat |
| 文献引用反应性 | mouse |
| 免疫原 | CCT2 fusion protein Ag19264 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chaperonin containing TCP1, subunit 2 (beta) |
| 别名 | 99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta |
| 计算分子量 | 535 aa, 57 kDa |
| 观测分子量 | 50-58 kDa |
| GenBank蛋白编号 | BC113516 |
| 基因名称 | CCT2 |
| Gene ID (NCBI) | 10576 |
| RRID | AB_2935302 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P78371 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCT2 antibody 68214-1-Ig | Download protocol |
| IF protocol for CCT2 antibody 68214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |