CoraLite® Plus 594-conjugated CCT2 Monoclonal antibody
CCT2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 594 Fluorescent Dye
CloneNo.
2H6C7
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy CCT2 Ready-To-Use IHC Kit. CCT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68214-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-68214 targets CCT2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Immunogen | CCT2 fusion protein Ag19264 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | chaperonin containing TCP1, subunit 2 (beta) |
| Synonyms | PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta |
| Calculated Molecular Weight | 535 aa, 57 kDa |
| Observed Molecular Weight | 50-58 kDa |
| GenBank Accession Number | BC113516 |
| Gene Symbol | CCT2 |
| Gene ID (NCBI) | 10576 |
| Conjugate | CoraLite® Plus 594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 594 nm / 615 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P78371 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 594 CCT2 antibody CL594-68214 | Download protocol |
| FC protocol for CL Plus 594 CCT2 antibody CL594-68214 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
