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验证数据展示

  • FC experiment of HepG2 using CL488-67400

    FC experiment of HepG2 using CL488-67400

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CCT5 (CL488-67400, Clone:1B12E10) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-67400

    IF Staining of HepG2 using CL488-67400

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CCT5 antibody (CL488-67400, Clone: 1B12E10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CCT5 Monoclonal antibody

CCT5 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67400

产品说明书

CloneNo. 1B12E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67400-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67400 targets CCT5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CCT5 fusion protein Ag21421 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 5 (epsilon)
别名 KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon
计算分子量 541 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC035499
基因名称 CCT5
Gene ID (NCBI) 22948
RRIDAB_3084344
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48643
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CCT5 antibody CL488-67400Download protocol
FC protocol for CL Plus 488 CCT5 antibody CL488-67400Download protocol
Standard Protocols
Click here to view our Standard Protocols