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验证数据展示

  • WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

    WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67540-1-Ig (same clone as 67540-1-PBS)

    IHC staining of mouse liver using 67540-1-Ig (same clone as 67540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 67540-1-Ig (same clone as 67540-1-PBS)

    IHC staining of mouse kidney using 67540-1-Ig (same clone as 67540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

    WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

    WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TCP1 eta Monoclonal antibody, PBS Only

TCP1 eta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67540-1-PBS

产品说明书

CloneNo. 2C1G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CCT7, CCT ETA, CCT-eta, CCTH, Chaperonin containing T-complex polypeptide 1 subunit 7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67540-1-PBS targets TCP1 eta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TCP1 eta fusion protein Ag9502 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 7 (eta)
别名 CCT7, CCT ETA, CCT-eta, CCTH, Chaperonin containing T-complex polypeptide 1 subunit 7
计算分子量 543 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC019296
基因名称 CCT7
Gene ID (NCBI) 10574
RRIDAB_2882759
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99832
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.