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  • Featured Product
  • KD/KO Validated

CCT7 Monoclonal antibody, PBS Only

CCT7 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

2C1G1

Cat No : 67540-1-PBS

Print datasheet

Synonyms

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta

验证数据展示

  • WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

    WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

    WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

    WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

产品信息

67540-1-PBS targets CCT7 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse
Immunogen CCT7 fusion protein Ag9502 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 7 (eta)
Synonyms CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta
Calculated Molecular Weight 543 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC019296
Gene Symbol CCT7
Gene ID (NCBI) 10574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99832
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.