验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, NIH/3T3 cells, HEK-293 cells, LNCaP cells, Hela cells, HepG cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | U2OS cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67540-1-Ig targets TCP1 eta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | TCP1 eta fusion protein Ag9502 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | chaperonin containing TCP1, subunit 7 (eta) |
| Synonyms | CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH |
| Calculated Molecular Weight | 543 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC019296 |
| Gene Symbol | CCT7 |
| Gene ID (NCBI) | 10574 |
| RRID | AB_2882759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99832 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCP1 eta antibody 67540-1-Ig | Download protocol |
| IF protocol for TCP1 eta antibody 67540-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
