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验证数据展示

  • IF Staining of U2OS using CL594-67540

    IF Staining of U2OS using CL594-67540

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®594 CCT7 antibody (CL594-67540, Clone: 2C1G1 ) at dilution of 1:200.

  • FC experiment of U2OS using CL594-67540

    FC experiment of U2OS using CL594-67540

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated CCT7 Monoclonal antibody (CL594-67540, Clone:2C1G1)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated TCP1 eta Monoclonal antibody

TCP1 eta Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67540

产品说明书

CloneNo. 2C1G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67540-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67540 targets TCP1 eta in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 TCP1 eta fusion protein Ag9502 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 7 (eta)
别名 CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH
计算分子量 543 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC019296
基因名称 CCT7
Gene ID (NCBI) 10574
RRIDAB_2934773
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99832
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

实验方案

Product Specific Protocols
IF protocol for CL594 TCP1 eta antibody CL594-67540Download protocol
FC protocol for CL594 TCP1 eta antibody CL594-67540Download protocol
Standard Protocols
Click here to view our Standard Protocols