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验证数据展示

  • IF Staining of U2OS using CL488-67540

    IF Staining of U2OS using CL488-67540

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 CCT7 antibody (CL488-67540, Clone: 2C1G1 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-67540

    FC experiment of U2OS using CL488-67540

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CCT7 Monoclonal antibody (CL488-67540, Clone:2C1G1)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TCP1 eta Monoclonal antibody

TCP1 eta Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67540

Print datasheet

CloneNo. 2C1G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67540-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67540 targets TCP1 eta in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen TCP1 eta fusion protein Ag9502 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 7 (eta)
Synonyms CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH
Calculated Molecular Weight 543 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC019296
Gene Symbol CCT7
Gene ID (NCBI) 10574
RRIDAB_2934550
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99832
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TCP1 eta antibody CL488-67540Download protocol
FC protocol for CL Plus 488 TCP1 eta antibody CL488-67540Download protocol
Standard Protocols
Click here to view our Standard Protocols