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CoraLite®594-conjugated CCT8 Monoclonal antibody

CCT8 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A7C3

Cat No : CL594-67539

Print datasheet

Synonyms

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

验证数据展示

  • IF Staining of MCF-7 using CL594-67539

    IF Staining of MCF-7 using CL594-67539

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 CCT8 antibody (CL594-67539, Clone: 1A7C3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67539-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67539 targets CCT8 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen CCT8 fusion protein Ag30082 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 8 (theta)
Synonyms C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta
Calculated Molecular Weight 548 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC012584
Gene Symbol CCT8
Gene ID (NCBI) 10694
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50990
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.

实验方案

Product Specific Protocols
IF protocol for CL594 CCT8 antibody CL594-67539Download protocol
FC protocol for CL594 CCT8 antibody CL594-67539Download protocol
Standard Protocols
Click here to view our Standard Protocols