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PE Anti-Human CD106 (1.G11B1)

CD106 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

1.G11B1

Cat no : PE-65049

Print datasheet

Synonyms

CD106, DKFZp779G2333, INCAM 100, V CAM 1, VCAM 1, VCAM1

验证数据展示

  • FC experiment of HUVEC using PE-65049

    FC experiment of HUVEC using PE-65049

    1X10^6 TNF-α stimulated HUVEC cells were surface stained with 10 ul PE Anti-Human CD106 (PE-65049, Clone:1.G11B1) (red) or PE-mouse IgG1 isotype control (black). Cells were not fixed.

经过测试的应用

Positive FC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65049 targets CD106 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name vascular cell adhesion molecule 1
Synonyms CD106, DKFZp779G2333, INCAM 100, V CAM 1, VCAM 1, VCAM1
Calculated Molecular Weight 739 aa, 81 kDa
GenBank Accession NumberBC017276
Gene Symbol VCAM-1
Gene ID (NCBI) 7412
RRIDAB_2883848
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP19320
Storage Buffer PBS with 0.1% sodium azide and a stabilizer.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
FC protocol for PE CD106 antibody PE-65049Download protocol
Standard Protocols
Click here to view our Standard Protocols