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APC Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Conjugate

APC Fluorescent Dye

CloneNo.

ABL-93

Cat no : APC-65052

Print datasheet

Synonyms

ABL93, CD107b, Lamp2, Mac3, Lamp 2

验证数据展示

  • IF Staining of NIH/3T3 using APC-65052

    IF Staining of NIH/3T3 using APC-65052

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using APC-65052 (CD107b antibody) at dilution of 1:100.

  • FC experiment of NIH/3T3 using APC-65052

    FC experiment of NIH/3T3 using APC-65052

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug APC Anti-Mouse CD107b / LAMP2 (APC-65052, Clone:ABL-93) or 0.25 ug APC Rat IgG2a Isotype Control (2A3) (APC-65209, Clone: 2A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65052 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Glycoproteins purified from BALB/c mouse embryo 3T3 cell line 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms ABL93, CD107b, Lamp2, Mac3, Lamp 2
GenBank Accession NumberBC138718
Gene Symbol CD107b / LAMP2
Gene ID (NCBI) 16784
RRIDAB_2882956
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP17047
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
IF protocol for APC CD107b / LAMP2 antibody APC-65052Download protocol
FC protocol for APC CD107b / LAMP2 antibody APC-65052Download protocol
Standard Protocols
Click here to view our Standard Protocols