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FITC Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b / LAMP2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

FITC Fluorescent Dye

CloneNo.

ABL-93

Cat no : FITC-65052

Print datasheet

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

验证数据展示

  • FC experiment of NIH/3T3 using FITC-65052

    FC experiment of NIH/3T3 using FITC-65052

    1X10^6 NIH/3T3 cells were intracellularly stained with 2 ul FITC Anti-Mouse CD107b (FITC-65052, Clone:ABL-93) (red), or 2 ul Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65052 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Mouse
Immunogen Glycoproteins purified from BALB/c mouse embryo 3T3 cell line 种属同源性预测
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3
GenBank Accession NumberBC138718
Gene Symbol CD107b
Gene ID (NCBI) 16784
RRIDAB_2883742
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths494 nm / 520 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP17047
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
FC protocol for FITC CD107b / LAMP2 antibody FITC-65052Download protocol
Standard Protocols
Click here to view our Standard Protocols