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CD107b / LAMP2 Polyclonal antibody

CD107b / LAMP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 32148-1-AP

Print datasheet

Synonyms

Lamp2, CD107b, Mac3, Lamp 2

验证数据展示

  • WB analysis using 32148-1-AP

    WB analysis using 32148-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32148-1-AP (CD107b / LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32148-1-AP targets CD107b / LAMP2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms Lamp2, CD107b, Mac3, Lamp 2
Calculated Molecular Weight46 kDa
Observed Molecular Weight70-100 kDa
GenBank Accession NumberNM_001017959.2
Gene Symbol CD107b / LAMP2
Gene ID (NCBI) 16784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP17047-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP2 is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 32148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols