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验证数据展示

  • FC experiment of human PBMCs using PE-65160

    FC experiment of human PBMCs using PE-65160

    1X10^6 human PBMCs were surface stained with 10 ul PE Anti-Human CD116 (PE-65160, Clone:4H1) (red), or PE Mouse IgG1 Isotype Control (blue), or unstained (black). Cells were not fixed. Monocytes were gated.

PE Anti-Human CD116 (4H1)

CD116 Monoclonal Antibody for FC
Cat No. PE-65160

产品说明书

CloneNo. 4H1

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

CD116, CDw116, CSF2R, CSF2RA, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM CSF R alpha, GMCSFR, GMCSFR alpha, GMR, GMR alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 μl per 10^6 cells in a 100 µl suspension or 10 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65160 targets CD116 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 CD116-transfected COS cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
别名 CD116, CDw116, CSF2R, CSF2RA, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM CSF R alpha, GMCSFR, GMCSFR alpha, GMR, GMR alpha
计算分子量 46 kDa
GenBank蛋白编号BC002635
基因名称 CD116
Gene ID (NCBI) 1438
RRIDAB_2883938
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP15509
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for PE CD116 antibody PE-65160Download protocol
Standard Protocols
Click here to view our Standard Protocols