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CoraLite®594-conjugated CD11a/Integrin Alpha L Monoclonal antibody

CD11a/Integrin Alpha L Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E3H12

Cat no : CL594-66256

Print datasheet

Synonyms

CD11A, CD11a/Integrin Alpha L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

验证数据展示

  • IF Staining of human tonsillitis using CL594-66256

    IF Staining of human tonsillitis using CL594-66256

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-66256

    IF Staining of human tonsillitis using CL594-66256

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66256-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66256 targets CD11a/Integrin Alpha L in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen CD11a/Integrin Alpha L fusion protein Ag7813 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Synonyms CD11A, CD11a/Integrin Alpha L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
RRIDAB_2919972
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20701
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
IF protocol for CL594 CD11a/Integrin Alpha L antibody CL594-66256Download protocol
Standard Protocols
Click here to view our Standard Protocols