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验证数据展示

  • FC experiment of human PBMCs using CL405-65116

    FC experiment of human PBMCs using CL405-65116

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11b (CL405-65116, Clone: ICRF44) or unstained. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL405-65116

    FC experiment of human PBMCs using CL405-65116

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11b (CL405-65116, Clone: ICRF44) or unstained. Cells were not fixed. Monocytes were gated.

CoraLite® Plus 405 Anti-Human CD11b (ICRF44)

CD11b Monoclonal Antibody for FC
Cat No. CL405-65116

产品说明书

CloneNo. ICRF44

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 405
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65116-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65116 targets CD11b in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha M (complement component 3 receptor 3 subunit)
别名 CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6
计算分子量 1152 aa, 127 kDa
GenBank蛋白编号BC096346
基因名称 CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
RRIDAB_2934355
偶联类型 CoraLite® Plus 405 Fluorescent Dye
最大激发/发射波长399 nm / 422 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP11215
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD11b antibody CL405-65116Download protocol
Standard Protocols
Click here to view our Standard Protocols