CoraLite® Plus 488 Anti-Human CD11b (ICRF44)
CD11b Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
ICRF44
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | human peripheral blood mononuclear cells |
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65116-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65116 targets CD11b in IF/ICC, FC applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC, FC Application Description |
| Tested Reactivity | Human |
| Immunogen | Rheumatoid synovial cells and human monocytes 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Synonyms | CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6 |
| Calculated Molecular Weight | 1152 aa, 127 kDa |
| GenBank Accession Number | BC096346 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 3684 |
| ENSEMBL Gene ID | ENSG00000169896 |
| RRID | AB_2883188 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P11215 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD11b antibody CL488-65116 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
