验证数据展示
经过测试的应用
Positive IF/ICC detected in | human peripheral blood mononuclear cells |
Positive FC detected in | human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-65116 targets CD11b in IF/ICC, FC applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC Application Description |
经测试反应性 | human |
免疫原 | Rheumatoid synovial cells and human monocytes 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | integrin, alpha M (complement component 3 receptor 3 subunit) |
别名 | ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha |
计算分子量 | 1152 aa, 127 kDa |
GenBank蛋白编号 | BC096346 |
基因名称 | CD11b |
Gene ID (NCBI) | 3684 |
ENSEMBL Gene ID | ENSG00000169896 |
RRID | AB_2883188 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P11215 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD11b antibody CL488-65116 | Download protocol |
FC protocol for CL Plus 488 CD11b antibody CL488-65116 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |