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CoraLite® Plus 488 Anti-Human CD11c (BU15)

CD11c Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

BU15

Cat no : CL488-65196

Print datasheet

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

验证数据展示

  • FC experiment of Human peripheral blood granulocytes using CL488-65196

    FC experiment of Human peripheral blood granulocytes using CL488-65196

    1X10^6 Human peripheral blood granulocytes were surface stained with 5 ul CoraLite® Plus 488-conjugated Anti-Human CD11c (CL488-65196, Clone: BU15) (green) or isotype control antibody (black). Cells were not fixed.

经过测试的应用

Positive FC detected inHuman peripheral blood granulocytes
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65196-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65196 targets CD11c in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human synovial fluid dendritic cells 种属同源性预测
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Synonyms CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6
Calculated Molecular Weight 1169 aa, 129 kDa
GenBank Accession NumberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2919270
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20702
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD11c antibody CL488-65196Download protocol
Standard Protocols
Click here to view our Standard Protocols