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Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody

CD122/IL-2RB Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241677H7

Cat no : 98151-1-RR

Print datasheet

Synonyms

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

验证数据展示

  • FC experiment of human PBMCs using 98151-1-RR

    FC experiment of human PBMCs using 98151-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (98151-1-RR, Clone: 241677H7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody (CL647-98033, Clone: 240078A7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98151-1-RR

    FC experiment of human PBMCs using 98151-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (98151-1-RR, Clone: 241677H7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98151-1-RR

    Affinity and Kinetic Characterization of 98151-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98151-1-RR against Human CD122/IL-2RB were performed. The affinity constant is 0.645 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98151-1-RR targets CD122/IL-2RB in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, beta
Synonyms IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta
Calculated Molecular Weight 551 aa, 61 kDa
GenBank Accession NumberBC025691
Gene Symbol IL2RB
Gene ID (NCBI) 3560
ENSEMBL Gene IDENSG00000100385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP14784
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor-mediated endocytosis and transduces the mitogenic signals of interleukin 2.

实验方案

Product Specific Protocols
FC protocol for CD122/IL-2RB antibody 98151-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols