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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 23457-1-AP (same clone as 23457-1-PBS)

    WB analysis using 23457-1-AP (same clone as 23457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    IP experiment of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    IP result of anti-IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:500) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CD126/IL-6R alpha Polyclonal antibody, PBS Only

CD126/IL-6R alpha Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 23457-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CD126, IL-6R alpha, IL 6R subunit alpha, IL 6R alpha, IL 6R 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

23457-1-PBS targets CD126/IL-6R alpha in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CD126/IL-6R alpha fusion protein Ag18263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 6 receptor
Synonyms CD126, IL-6R alpha, IL 6R subunit alpha, IL 6R alpha, IL 6R 1
Calculated Molecular Weight 468 aa, 52 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC132684
Gene Symbol IL-6R alpha
Gene ID (NCBI) 3570
RRIDAB_2827428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).