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Anti-Mouse IL-7Ra/CD127 (A7R34)

IL-7Ra/CD127 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

A7R34

Cat no : 65093-1-Ig

Print datasheet

Synonyms

CD127, Il7r, Il-7r, IL- 7Ralpha, IL 7Ralpha

验证数据展示

  • FC experiment of mouse splenocytes using 65093-1-Ig

    FC experiment of mouse splenocytes using 65093-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD127 (IL-7Ra) (65093-1-Ig, Clone: A7R34) or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65093-1-Ig targets IL-7Ra/CD127 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen IL-7Ra-IgG1 fusion protein 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 7 receptor
Synonyms CD127, Il7r, Il-7r, IL- 7Ralpha, IL 7Ralpha
GenBank Accession NumberBC089571
Gene Symbol CD127
Gene ID (NCBI) 16197
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP16872
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

实验方案

Product Specific Protocols
FC protocol for IL-7Ra/CD127 antibody 65093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols