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FITC Plus Anti-Mouse IL-7Ra/CD127 (A7R34)

IL-7Ra/CD127 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

A7R34

Cat no : FITC-65093

Print datasheet

Synonyms

CD127, Il7r, Il-7r, IL- 7Ralpha, IL 7Ralpha

验证数据展示

  • FC experiment of mouse splenocytes using FITC-65093

    FC experiment of mouse splenocytes using FITC-65093

    1x10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD127 (IL-7Ra) (A7R34) (FITC-65093, Clone: A7R34) or 0.5 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were co-stained with Anti-Mouse CD3. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65093 targets IL-7Ra/CD127 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen IL-7Ra-IgG1 fusion protein 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 7 receptor
Synonyms CD127, Il7r, Il-7r, IL- 7Ralpha, IL 7Ralpha
GenBank Accession NumberBC089571
Gene Symbol CD127
Gene ID (NCBI) 16197
RRIDAB_2883773
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP16872
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

实验方案

Product Specific Protocols
FC protocol for FITC Plus IL-7Ra/CD127 antibody FITC-65093Download protocol
Standard Protocols
Click here to view our Standard Protocols