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Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody

CD13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

WM15

Cat no : 65633-1-MR

Print datasheet

Synonyms

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

验证数据展示

  • FC experiment of human PBMCs using 65633-1-MR

    FC experiment of human PBMCs using 65633-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody (65633-1-MR, Clone: WM15) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65633-1-MR

    FC experiment of human PBMCs using 65633-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody (65633-1-MR, Clone: WM15) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. Monocytes were gated.

  • IF Staining of THP-1 using 65633-1-MR

    IF Staining of THP-1 using 65633-1-MR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65633-1-MR, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive IF/ICC detected inTHP-1 cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD13 Ready-To-Use IHC Kit. CD13 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65633-1-MR targets CD13 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity human
Immunogen Human AML cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name alanyl (membrane) aminopeptidase
Synonyms APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC058928
Gene Symbol CD13
Gene ID (NCBI) 290
ENSEMBL Gene IDENSG00000166825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

实验方案

Product Specific Protocols
IF protocol for CD13 antibody 65633-1-MRDownload protocol
FC protocol for CD13 antibody 65633-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols