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验证数据展示

  • FC experiment of human PBMCs using 65633-1-MR (same clone as 65633-1-PBS)

    FC experiment of human PBMCs using 65633-1-MR (same clone as 65633-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a RecAb (65633-1-MR, Clone:WM15) and PE-conjugated Goat Anti-Mouse IgG, or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65633-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65633-1-MR (same clone as 65633-1-PBS)

    FC experiment of human PBMCs using 65633-1-MR (same clone as 65633-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a RecAb (65633-1-MR, Clone: WM15) (red) and PE-conjugated Goat Anti-Mouse IgG, or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65633-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 65633-1-MR (same clone as 65633-1-PBS)

    IF Staining of THP-1 using 65633-1-MR (same clone as 65633-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65633-1-MR, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 65633-1-PBS in a different storage buffer formulation.

Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody, PBS Only

CD13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 65633-1-PBS

产品说明书

CloneNo. WM15

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC

别名

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD13 Ready-To-Use IHC Kit. CD13 primary antibody included.

产品信息

65633-1-PBS targets CD13 in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原 Human AML cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 alanyl (membrane) aminopeptidase
别名 APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M
计算分子量 110 kDa
GenBank蛋白编号BC058928
基因名称 CD13
Gene ID (NCBI) 290
ENSEMBL Gene IDENSG00000166825
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)