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Anti-Human CD134/OX40 Rabbit Recombinant Antibody

CD134/OX40 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241398G7

Cat no : 98181-1-RR

Print datasheet

Synonyms

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

验证数据展示

  • FC experiment of human PBMCs using 98181-1-RR

    FC experiment of human PBMCs using 98181-1-RR

    1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were not fixed.

  • FC experiment of human PBMCs using 98181-1-RR

    FC experiment of human PBMCs using 98181-1-RR

    1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98181-1-RR

    Affinity and Kinetic Characterization of 98181-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98181-1-RR against Human CD134/OX40 were performed. The affinity constant is 5.48 nM.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98181-1-RR targets CD134/OX40 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 4
Synonyms TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen
Calculated Molecular Weight 29 kDa
GenBank Accession NumberNM_003327
Gene Symbol CD134
Gene ID (NCBI) 7293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43489
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

实验方案

Product Specific Protocols
FC protocol for CD134/OX40 antibody 98181-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols