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Anti-Human CD137 (4B4-1)

CD137 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

4B4-1

Cat No : 65159-1-Ig

Print datasheet

Synonyms

TNFRSF9, T cell antigen 4 1BB homolog, ILA, 4B4-1, 4-1BB ligand receptor

验证数据展示

  • FC experiment of human peripheral blood mononuclear cells using 65159-1-Ig

    FC experiment of human peripheral blood mononuclear cells using 65159-1-Ig

    1X10^6 PHA stimulated (5 μg/mL, 3 days) (right) or unstimulated (left) human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.13 ug Anti-Human CD137 (65159-1-Ig, Clone: 4B4-1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood mononuclear cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65159-1-Ig targets CD137 in FC applications and shows reactivity with human, non-human primates samples.

Tested Applications FC Application Description
Tested Reactivity human, non-human primates
Immunogen Recombinant human CD137 ectodomain 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 9
Synonyms TNFRSF9, T cell antigen 4 1BB homolog, ILA, 4B4-1, 4-1BB ligand receptor
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC006196
Gene Symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
RRIDAB_2918441
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDQ07011
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

实验方案

Product Specific Protocols
FC protocol for CD137 antibody 65159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols