Back

Mouse IgG1 isotype control Monoclonal antibody 1 Publications

Mouse Monoclonal| Catalog number: 66360-1-Ig |CloneNo.: 1F8D3

浓度: 1000 μg/ml

点击咨询在线客服

Applications

Tested Applications:
FC, WB,ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IP


Images See all

FC experiment of K-562 using 66360-1-Ig

1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.

WB analysis using 66360-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB analysis using 66360-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Species Specificity:
Megathura crenulata
Positive WB detected in:
Positive FC detected in:
Recommended dilution:
WB : 1:500-1:2000

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
Mouse IgG1 isotype control
Calculated molecular weight:
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms
Kappa light chain, KLH, Mouse IgG1 isotype control

Background

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

PRODUCT SPECIFIC PROTOCOLS

STANDARD PROTOCOLS

Click here to view our Standard Protocols

Back
to top