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CD138/Syndecan-1 Monoclonal antibody, PBS Only (Capture)

CD138/Syndecan-1 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6A2

Cat no : 60185-1-PBS

Print datasheet

Synonyms

CD138, 1B6A2, syndecan, SYND1, SDC1

验证数据展示

  • WB analysis of BxPC-3 using 60185-1-Ig (same clone as 60185-1-PBS)

    WB analysis of BxPC-3 using 60185-1-Ig (same clone as 60185-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60185-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60185-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50044-1

    Sandwich ELISA standard curve of MP50044-1

    Sandwich ELISA standard curve of MP50044-1, SDC1,CD138 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60185-1-PBS. Detection antibody: 67155-1-PBS. Standard: Ag17733. Range: 0.188-12 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.

产品信息

60185-1-PBS targets CD138/Syndecan-1 as part of a matched antibody pair:

MP50044-1: 60185-1-PBS capture and 67155-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name syndecan 1
Synonyms CD138, 1B6A2, syndecan, SYND1, SDC1
Calculated Molecular Weight 32.5 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC008765
Gene Symbol CD138
Gene ID (NCBI) 6382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18827
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.