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验证数据展示

  • FC experiment of human PBMCs using 98040-1-RR

    FC experiment of human PBMCs using 98040-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98040-1-RR

    FC experiment of human PBMCs using 98040-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98040-1-RR

    Affinity and Kinetic Characterization of 98040-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98040-1-RR against Human CD14 were performed. The affinity constant is 0.703 nM.

Anti-Human CD14 Rabbit Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98040-1-RR

产品说明书

CloneNo. 230332D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

230332D7, CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inFC receptor blocked human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98040-1-RR targets CD14 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD14 molecule
别名 230332D7, CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_3672187
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08571
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for CD14 antibody 98040-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols