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Anti-Human CD14 Rabbit Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230332D7

Cat no : 98040-1-RR

Print datasheet

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D7

验证数据展示

  • FC experiment of human PBMCs using 98040-1-RR

    FC experiment of human PBMCs using 98040-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98040-1-RR

    FC experiment of human PBMCs using 98040-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7)(red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98040-1-RR

    Affinity and Kinetic Characterization of 98040-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98040-1-RR against Human CD14 were performed. The affinity constant is 0.703 nM.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98040-1-RR is CD14 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD14 molecule
Synonyms Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D7
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08571
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for CD14 antibody 98040-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols